What Is Insulin Analog, Lispro Mixed? Uses, Dosage, and What You Need to Know in 2026

Insulin Analog, Lispro Mixed is a combination insulin product used to manage blood sugar in adults with Type 1 or Type 2 diabetes. Sold under the Humalog Mix brand name in two formulations — 75/25 and 50/50 — it combines a rapid-acting and an intermediate-acting insulin analog in a single injection. This guide covers everything you need to know about what it is, how it works, how to use it, and what to watch out for.

What Is Insulin Analog, Lispro Mixed?

Insulin Analog, Lispro Mixed is the generic name for a premixed insulin that combines two forms of insulin lispro:

• Insulin lispro (rapid-acting): Works within 10–20 minutes to cover mealtime blood sugar rises
• Insulin lispro protamine (intermediate-acting): Provides background blood sugar control for up to 24 hours

The two formulations available are:

• Humalog Mix 75/25: 75% insulin lispro protamine (intermediate) and 25% insulin lispro (rapid)
• Humalog Mix 50/50: 50% insulin lispro protamine (intermediate) and 50% insulin lispro (rapid)

Both are manufactured by Eli Lilly and Company and are injected under the skin (subcutaneously). They are available as 10 mL vials and KwikPens (prefilled injection pens).

What Is Insulin Analog, Lispro Mixed Used For?

Insulin Analog, Lispro Mixed is FDA-approved to improve blood sugar (glycemic) control in adults with diabetes mellitus — both Type 1 (insulin-dependent) and Type 2 (non-insulin-dependent). It is not approved for use in patients younger than 18 years old.

The 75/25 formulation is the most commonly prescribed version and is typically used in patients whose meal timing is consistent and who benefit from a twice-daily injection schedule that covers both mealtime and background needs.

How Do You Take Insulin Analog, Lispro Mixed?

This medication is injected under the skin (subcutaneously). It should be injected within 15 minutes before a meal — not after, and not hours before. The typical schedule is twice daily: before breakfast and before the evening meal.

Approved injection sites: Abdomen (fastest absorption), upper arm, thigh, or buttocks. Rotate sites with each injection.

How to prepare the suspension: Roll the vial or pen gently between your palms 10 times, then tip it up and down 10 times to mix. The solution should look cloudy and white — do not use it if it is clear, has clumps, or has a frosted appearance. Never shake it vigorously.

Cannot be used in insulin pumps: Insulin Analog, Lispro Mixed is a suspension and is not suitable for continuous subcutaneous insulin infusion devices (insulin pumps).

What Is the Typical Dose?

Dosing is highly individualized. Your doctor will determine your starting dose based on your weight, blood sugar readings, eating habits, activity level, and other medications. Typical daily insulin requirements for adults range between 0.5 and 1 unit per kilogram of body weight — but your dose may be significantly different. Never change your dose without consulting your prescriber.

How Should Insulin Analog, Lispro Mixed Be Stored?

Unopened vials and pens: Refrigerate at 36–46°F (2–8°C). Do not freeze. If stored at room temperature, use before expiration.

Open vials: Can be stored at room temperature or refrigerator — use within 28 days.

Open KwikPens: Store at room temperature only (do not refrigerate opened pens) — use within 10 days. Do not store with needle attached.

Who Should Not Use Insulin Analog, Lispro Mixed?

Do not use this medication if you are allergic to insulin lispro or any ingredient in the formulation, or if you are currently experiencing hypoglycemia (low blood sugar). This medication is not approved for children under 18. Tell your doctor about all medications, supplements, and health conditions before starting.

The Bottom Line

Insulin Analog, Lispro Mixed is a reliable, well-studied insulin product that millions of people with diabetes use to manage their blood sugar. If you are having trouble finding it at your pharmacy, medfinder can check nearby pharmacies on your behalf. For a deeper dive into how this medication works at a biological level, see: How Does Insulin Analog, Lispro Mixed Work? Mechanism of Action Explained.